Dnak gene
WebJun 3, 2009 · The heat shock protein 70 (Hsp70/DnaK) gene of Bacillus licheniformis is 1,839 bp in length encoding a polypeptide of 612 amino acid residues. The deduced amino acid sequence of the gene shares high sequence identity with other Hsp70/DnaK proteins. The characteristic domains typical for Hsps/DnaKs are also well conserved in B. … WebThe average sequence similarity for the dnaK gene (87.8%) among type strains was significantly less than that of the 16S rRNA sequence (99.1%). Therefore, the dnaK gene …
Dnak gene
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WebJan 23, 2007 · Function. Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release … WebHyaluronic acid (HA) is a multiple-function biopolymer that is widely used in food, cosmetic, and biomedical fields. In group C streptococci, the major workhorse of HA production in industry, the HA biosynthetic pathway has been proposed, while how HA synthesis is regulated is unclear. In this study, we identified twenty-five putative transcriptional …
WebMar 15, 1994 · Eukaryotic organisms have been shown to have multiple forms of hsp70-class stress-related proteins, but only a single family member, DnaK, has been found in … WebThe expression of the dnaK gene in the cyanobacterium Synechocystis sp. strain PCC 6803 was continuously monitored as bioluminescence by an automated monitoring system, using the bacterial luciferase genes (luxAB) of Vibrio harveyi as a reporter of promoter activity. A dnaK-reporting bioluminescent …
WebJun 23, 2024 · The PCR amplification of the 16S rRNA, housekeeping genes (atpD, dnaK, glnll and rpoB) and symbiotic genes (nifH and nodC) were individually performed in a 25 µL PCR reaction volume, which ... WebMar 15, 1994 · Eukaryotic organisms have been shown to have multiple forms of hsp70-class stress-related proteins, but only a single family member, DnaK, has been found in prokaryotes. We report here the identification of a heat shock cognate gene, designated hsc, in Escherichia coli. The amino acid sequence deduced from hsc predicts a 65,647-Da …
WebApr 15, 2024 · The drought-responsive transcripts formed co-expression gene modules and were majorly associated with redox pathways and carbohydrate metabolism. ... The cytochrome P450 was up-regulated under drought stress but DnaK was down-regulated under drought stress, in Chandan. Table 1 Candidate stress-tolerant genes, selected for …
WebJan 1, 2015 · The DnaK gene of A. acidoterrestris was sequenced and the bioinformatics analyses indicated thermostable protein structures of DnaK protein.. The mRNA levels of … streamlined forensic reportingWebMar 1, 2014 · The dnak gene from one representative, Bacillus pumilus strain B3 isolated from Bakreshwar hot springs was successfully cloned and sequenced. The dnaK gene … streamlined in a sentenceWebThe expression of the dnaK gene in the cyanobacterium Synechocystis sp. strain PCC 6803 was continuously monitored as bioluminescence by an automated monitoring system, … streamlined forensic reporting toolkitWebDec 22, 1998 · Random mutagenesis of the entire dnaK gene was performed by PCR with Taq DNA polymerase by using a 5′-primer introduced AflIII site that is compatible with NcoI site for ligation and a 3′-primer introduced BamHI site.The PCR reactions contained 10 mM Tris⋅HCl (pH 8.3), 50 mM KCl, 10 ng of pNRK416, 50 pmol of each primer, 0.2 mM … streamlined forensic reporting limitedWebThe bacterial molecular chaperone DnaK is an enzyme that couples cycles of ATP binding, hydrolysis, and ADP release by an N-terminal ATP-hydrolyzing domain to cycles of sequestration and release of unfolded proteins by a C-terminal substrate binding domain. Dimeric GrpE is the co-chaperone for DnaK, and acts as a nucleotide exchange factor ... streamlined foreign offshore procedures irsWebThe Escherichia coli DnaK heat shock protein has been identified previously as a negative regulator of E. coli heat shock gene expression. We report that two other heat shock … streamlined forensic digital evidence reportWebThe bacterial molecular chaperone DnaK is an enzyme that couples cycles of ATP binding, hydrolysis, and ADP release by an N-terminal ATP-hydrolyzing domain to cycles of … streamlined irs installment agreement