Flp-in cho
WebMar 22, 2024 · Cystic fibrosis (CF) is a genetic disease that affects the lungs and digestive system by making fluids and mucus thick and sticky. This causes persistent lung infections that progressively limit the ability to breathe. Children with CF also suffer from digestive problems that prevent their bodies from breaking down and absorbing food, leading ... WebFlp-In™ T-REx™-293 3 × 106 cells, frozen R780-07 Flp-In™ Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™ host cell lines that stably express the lacZ-Zeocin™ fusion gene from pFRT/lacZeo or pFRT/lacZeo2 (Flp-In™-CHO). Each cell line contains a single integrated FRT site
Flp-in cho
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WebJan 21, 2011 · The transport assay for other sphingolipids was performed with Flp-In-CHO/SPHK1 cells stably expressing each transporter as described previously. The … WebJan 15, 2024 · We also established CHO cells expressing the WT protein using the Flp-In system as previously described (thereafter called Flp-In CHO-WT cells) [6]. The pcDNA5/FRT-PiT-2-flag in GFP vectors were obtained by inserting GFP into Loop7 of the SLC20A2 (WT, T115 M, and R467X) using in-fusion cloning system (Takara Bio Inc.; …
WebFlp-In™Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™host cell lines that stably express the lacZ-Zeocin™fusion gene from pFRT/lacZeo or pFRT/lacZeo2. Each cell line contains a single integrated FRT site as confirmed by Southern blot analysis. WebAug 8, 2016 · MIL75-pFRT-FTMK and POG44 were co-transfected (1:9) into Flp-In-CHO cells. After 48 h of transfection, the cells were cultured under selection pressure by hygromycin B (1 mg/ml).
WebFRT site in the Flp-In™-CHO™ cell line has not been mapped, but has been demonstrated to have integrated into a highly transcriptionally active genomic locus as determined by … WebMar 5, 2024 · Idiopathic Basal Ganglia Calcification (IBGC) is a rare neuropsychiatric illness also known as Fahr's disease or Primary Familial Brain Calcification (PFBC). IBGC is caused by SLC20A2 variants, which encodes the inorganic phosphate (Pi) transporter PiT-2, a transmembrane protein associated with Pi homeostasis.
WebNov 11, 2013 · Flip-In CHO cells (FCHO) stably transfected by plasmid DNA were stained by PE-conjugated mouse-anti-human kappa chain antibodies (PE-Kappa) and then analyzed by FACS. ... Invitrogen (2006) Flp-In system for generating stable mammalian expression cell lines by Flp recombinase-mediated integration. Instruction Manual. Carlsbad, CA. 22.
WebA single gene copy of shRNA-2 also decreased the translational level of DHFR by 80% in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop … how many gun deaths in 2023WebSeveral Flp-In™ host cell lines which stably express the lacZ-Zeocin™ fusion gene and contain a single integrated FRT site are available (see page 7 for ordering information). To express your gene of interest in 293, CV-1, CHO, 3T3, BHK, or Jurkat cells, you may want to use one of the Flp-In™ host cell lines to establish how many gun deaths in germanyWebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each how a 2 way switch worksWebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably … how many gun deaths in chicago 2022WebA, assay for the evaluation of the interaction between cells and surface-bound collectins. Step 1: incubation of AF 488-conjugated bioparticles with collectins. Step 2: incubation of … howa 30-06 riflehow many gun deaths in chicago last weekendWebFeb 13, 2024 · Flp‐In CHO‐METKO cells (METKO), wild‐type MET (MET WT)–, or 11N all 11Q mutant MET (11N all 11Q)–transfected Flp‐In CHO‐METKO cells were stained with FITC‐conjugated anti‐MET antibody and analyzed by flow cytometry. C, Confocal microscopy analysis of MET (red) and nuclear (cyan). Flp‐In CHO‐METKO cells … how many gun deaths in germany in 2021