Osteoclast markers for fluorescent imaging

Author: pesp

August undefined, 2024

WebJun 15, 2024 · We have developed the fluorescent probe to monitor osteoclast activity, by the combination of pH sensitive fluorescent dye wi … For the clinical diagnosis of osteoporosis, X-ray CT and biochemical bone metabolism markers, however, there is no method to monitor osteoclast activity in the living system. WebDec 1, 2011 · To perform the live imaging of osteoclasts in vivo, we generated a medaka transgenic line in which the fluorescent proteins were expressed under the control of the TRAP (tartrate-resistant acid phosphatase) promoter (TRAP-GFP, Fig. 1A–D) or cathepsin …

Tobacco Mosaic Viral Nanoparticle Inhibited Osteoclastogenesis …

WebIn order to understand osteoclast cell biology, ... Kenworthy, A. K. (2001) Imaging protein-protein interactions using fluorescence resonance energy transfer ... Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., and Miyawaki, A. (2002) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl ... WebImage of the calvarial bone from CX3CR1-EGFP knock-in mice by intravital multi-photon imaging. CX3CR1-positive cells appear green. Blood vessels are stained by injecting Texas Red-conjugated 70 ... b2主题代码高亮

In Vivo Fluorescence Imaging of Bone-Resorbing Osteoclasts

WebThere has been a number of attempts to overcome the above limitations. Transgenic expression of the red fluorescent tdTomato protein under the control of the Acp5 promoter allowed the fluorescence-based real-time analysis of TRAP expression as a measure of … WebFeb 24, 2024 · The excitation wavelength of 940 nm is used to simultaneously excite ECFP, tdTomato, and pHocas-3. For an example of intravital time-lapse bone imaging, image stacks were collected at 3 μm vertical steps at a depth of 50–150 μm below the skull bone surface with 2.0× zoom, 512 × 512 X–Y resolution, and a time resolution of 5 min. 14. WebJun 26, 2024 · In Vivo Multicolor Imaging with Fluorescent Probes Revealed the Dynamics and Function of Osteoclast Proton Pumps ACS Cent Sci. 2024 Jun ... To our knowledge, this is the first study to demonstrate the direct involvement of osteoclast proton pumps in … b2下一代

Osteoclasts in bone modeling, as revealed by in vivo imaging, are ...

Quantitative Analysis of Osteoclast-Specific Gene Markers …

WebNormalizing bone metabolism is a challenge in rheumatoid arthritis (RA). Studies in mice suggest that anti-citrullinated protein antibodies (ACPAs) can trigger osteoclast activation and bone resorption in the bone marrow. However, data on the presence and role of ACPAs in human bone marrow are scarce. We investigated whether ACPAs can contribute to … WebMar 4, 2024 · Intravital imaging of osteoclast fission and cellular ... These markers may therefore be useful to identify osteomorphs in non-chimeric mice and humans. Osteomorph genes ... (Invitrogen, Carlsbad, CA, USA) or OsteoSense 680EX Fluorescent Imaging Agent (PerkinElmer Inc., Waltham, MA, USA), and Hoechst 33258 (bis-benzimide ... b2主题优惠WebVirus-based nanoparticles have been shown great potentials in biomedical applications such as drug delivery system, 1 imaging probe, 2 and ... and/or protein levels of osteoclastogenesis genes NFATc1 and RANK and osteoclast markers Ctr, Ctsk, Mmp-9, and Trap were ... fluorescent virus-like nanoparticles that represent a convenient tool ... b2上限

"WebOct 13, 2024 · PERK inhibitor GSK2606414 down-regulated the mRNA levels and protein expression of osteoclast differentiation marker ... by using Image J, Scale bar ... tandem fluorescent protein adenovirus to ... " - Osteoclast markers for fluorescent imaging

Osteoclast markers for fluorescent imaging

In Vivo Multicolor Imaging with Fluorescent Probes Revealed the ...

WebDisruptions of bone homeostasis accompany disorders that include osteoporosis, arthritis, and many inheritable skeletal diseases. R&D Systems offers products that can be used as cell-type specific markers for osteoblasts (i.e. osteocalcin, DMP-1, osteoahderin) and … WebNov 9, 2011 · These pK (a) values should be appropriate to perform selective imaging of bone-resorbing osteoclasts, because synthesized probes cannot fluoresce intrinsically at physiological pH and the pH in the resorption pit is lowered to about 4.5. Furthermore, …

Did you know?

WebIn order to understand osteoclast cell biology, ... Fluorescence imaging of osteoclasts using confocal microscopy Methods Mol Biol. 2012;816:401-24. doi: 10.1007/978-1-61779-415-5_25. Author Fraser P Coxon 1 ... Fluorescent Dyes / chemical synthesis WebJun 15, 2024 · We have developed the fluorescent probe to monitor osteoclast activity, by the combination of pH sensitive fluorescent dye wi … For the clinical diagnosis of osteoporosis, X-ray CT and biochemical bone metabolism markers, however, there is no …

WebJul 5, 2024 · Experimental design. Preliminary tests were conducted to determine whether fluorescent labeling methods can be used to study osteoclast fusion. To begin, live Raw264.7 cells were divided into two ...

WebFeb 10, 2024 · Similar to the reduction in osteoclast markers, we found significant reductions in overall resorption areas and in pit area per osteoclast in IgSF11 −/− cultures (Fig. 2c). WebMay 1, 2024 · Request PDF In Vivo Multicolor Imaging with Fluorescent Probes Revealed the Dynamics and Function of Osteoclast Proton Pumps In vivo two-photon fluorescence imaging is a powerful modality to ...

WebFeb 27, 2024 · Objective To describe a novel fluorescent histochemical protocol to visualize osteoclasts, vasculature, and nerves in thick sections of human osteochondral tissues and to demonstrate its feasibility for use in radiologic-pathologic research correlation studies. Materials and methods Consecutive patients scheduled for total knee arthroplasty …

WebDec 14, 2012 · Fluorescent Imaging Techniques. ... CD38 and Lrg1 in CIA, indicating that they could be used as disease markers. Moreover, osteoclast pathway-identifying genes remained similarly expressed ... b2主题首页美化WebJul 24, 2024 · For osteoclast markers, we found no significant difference of rank, acp5b and ctsk expression between WT and Tg(Ola.Sp7:N1aICD) cy31 (Figure 8B). It is also interesting to note that two genes correlated to bone remodeling, opn (osteopontin) and phex (phosphate-regulating neutral endopeptidase), were significantly downregulated in … b2b란 무엇인가WebJul 27, 2024 · We thank Georges Lutfalla (Université Montpellier) for sharing fluorescent E. coli and pTol2-mCherry-F plasmid and Baubak Bajoghli (University of Tuebingen) for providing the cxcr3.2-GFP plasmid. ... Live imaging of osteoclast inhibition by bisphosphonates in a medaka osteoporosis model. Dis. Model. Mech. 9, 155–163 (2016). … b2主题美化教程WebApr 13, 2024 · On day 5, the expression of nuclear factor of activated T cell 1 (NFATc1), acid phosphatase 5 (Acp5), cathepsin K (Ctsk) and matrix metalloprotein 9 (Mmp9), marker genes of osteoclast activity ... b2事件计算公式WebAug 1, 2006 · Download : Download full-size image; Figure 2. Expression of osteoclast specific marker mRNA. The amplification curves of quantitative real time RT-PCR analysis: TRAP (a), cathepsin K (b), and calcitonin receptor (c). Relative abundance of mRNA represents the fold-change in expression with respect to the control (group A) (= 1.0). dasa zukova instagramWebMar 25, 2024 · e Indirect fluorescent signals for osteoclast makers CAII, CR, CK, V ... These results suggest that expression of osteoclast markers induced by PEG treatment of PBMCs is transient as their expression rapidly increase between days 1 and 7 and then is followed … b2二进制WebMay 1, 2024 · Introduction. Osteoclasts are bone-resorbing cells closely related to bone turnover, whereas different macrophage subtypes contribute to bone fracture healing. As osteoclasts and macrophages share the same hematopoietic origin, the difference between both cell types on osteoblast coupling, crosstalk extent and consequent bone formation … dasaita max11 rav4 gps doesn\\u0027t work